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goat α mcherry  (OriGene)


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    Structured Review

    OriGene goat α mcherry
    Goat α Mcherry, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+mcherry/mCherry+Goat+Polyclonal+Antibody/bio_rxiv__64898__2026__03__19__712911-44-3-7
    Average 94 stars, based on 8 article reviews
    goat α mcherry - by Bioz Stars, 2026-11
    94/100 stars

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    Incubation:

    Article Title: Activation of orexin neurons changes reward-based decision-making strategies
    Article Snippet: .. Sections were incubated with primary antibody goat anti-orexin A (1:500 or 1,000, sc-8070, Santa Cruz Biotechnology) or rabbit anti-orexin A (1:2,000, Phoenix Pharmaceuticals, Belmont, CA, USA), rabbit anti-DsRed (1:1,000, Cat# 632496, TaKaRa Bio USA), goat anti-mCherry (1:1,000, AB0040, OriGene Technologies, Inc., MD, USA), rabbit anti-c-Fos (1:1,000, sc-253, Santa Cruz Biotechnology; 1:1,000, ab190289, abcam), and mouse anti-Cre (1:1,000, MAB3120, Millipore, Burlinton, MA, USA) followed by the appropriate secondary antibody, specifically donkey anti-goat or anti-rabbit immunoglobulin IgG (1:500 or 1,000, Alexa Fluor 488) or anti-rabbit or anti-mouse IgG (1:500 or 1,000, Alexa Fluor 546 or 594). .. Sections were also incubated with PureBlu DAPI Nuclear Staining Dye (#135-1303, Bio-Rad Laboratories, Hercules, CA, USA) or Hoechst 33342 (346-07951, 1:2,000, Dojindo, Kumamoto, Japan).

    Article Title: Presynaptic maturation of inhibitory connections onto vasoactive intestinal polypeptide-expressing GABAergic interneurons in the mouse barrel field
    Article Snippet: For free-floating staining, coronal trimmed slices of the somatosensory cortex were washed with phosphate-buffered saline (PBS) 0.01 M and then blocked and permeabilised for 2 h at room temperature (RT) with a solution containing 7% normal donkey serum (017–000–121, Dianova, Hamburg, Germany) and 0.8% Triton X-100 in 0.01 M PBS. .. The sections were incubated for 2 days at 4 °C in the primary antibodies (ABs) (guinea pig) anti-VGAT 1:500 (131004, Synaptic Systems, Göttingen, Germany) and (goat) anti-mCherry 1:100 (AB0040-200, Sicgen, OriGene Technology GmbH, Herford, Germany) in a staining buffer containing 2% bovine serum albumin (001–000–161, Jackson ImmunoResearch, Dianova, Germany) with 0.05% azide and 0.3% Triton X-100 in 0.01 M PBS. .. Slices were washed extensively with 0.01 M PBS and then incubated with the appropriate secondary antibodies and 0.5 μg/mL DAPI (A4099.0005, AppliChem, Darmstadt, Germany) for 2 h at RT in a staining solution containing 2% bovine serum albumin (001–000–161, Dianova) with 0.05% azide.


    Staining:

    Article Title: Artificially induced torpor during pregnancy impairs fetal growth in mice
    Article Snippet: .. The primers (5′–3′) used to produce RNA probes were as follows (the first one, forward primer, the second one, reverse primer): vGluT2 (part 1), 5′-TAGCTTCCTCTGTCCGTGGT; 5′-GGGCCAAAATCCTTTGTTTT vGluT2 (part 2), 5′-CCACCAAATCTTACGGTGCT; 5′-GGAGCATACCCCTCCCTTTA vGluT2 (part 3), 5′-CTCCCCCATTCACTACCTGA; 5′-GGTCAGGAGTGGTTTGCATT A mixture of parts 1–3 was used. mCherry was stained with goat anti-mCherry (AB0040-200; OriGene; 1:500) and anti-goat 555 (A32816; Invitrogen; 1:500). .. Fluoromount (K024; Diagnostic BioSystems) was used as the mounting medium.

    Article Title: Presynaptic maturation of inhibitory connections onto vasoactive intestinal polypeptide-expressing GABAergic interneurons in the mouse barrel field
    Article Snippet: For free-floating staining, coronal trimmed slices of the somatosensory cortex were washed with phosphate-buffered saline (PBS) 0.01 M and then blocked and permeabilised for 2 h at room temperature (RT) with a solution containing 7% normal donkey serum (017–000–121, Dianova, Hamburg, Germany) and 0.8% Triton X-100 in 0.01 M PBS. .. The sections were incubated for 2 days at 4 °C in the primary antibodies (ABs) (guinea pig) anti-VGAT 1:500 (131004, Synaptic Systems, Göttingen, Germany) and (goat) anti-mCherry 1:100 (AB0040-200, Sicgen, OriGene Technology GmbH, Herford, Germany) in a staining buffer containing 2% bovine serum albumin (001–000–161, Jackson ImmunoResearch, Dianova, Germany) with 0.05% azide and 0.3% Triton X-100 in 0.01 M PBS. .. Slices were washed extensively with 0.01 M PBS and then incubated with the appropriate secondary antibodies and 0.5 μg/mL DAPI (A4099.0005, AppliChem, Darmstadt, Germany) for 2 h at RT in a staining solution containing 2% bovine serum albumin (001–000–161, Dianova) with 0.05% azide.



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